{"id":2686,"date":"2026-05-04T04:50:50","date_gmt":"2026-05-04T04:50:50","guid":{"rendered":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/biotium-tech-tip-2\/"},"modified":"2026-05-04T04:50:51","modified_gmt":"2026-05-04T04:50:51","slug":"biotium-tech-tip-2","status":"publish","type":"post","link":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/biotium-tech-tip-2\/","title":{"rendered":"Biotium Tech Tip"},"content":{"rendered":"<figure class=\"project-feature\">\n<img decoding=\"async\" alt=\"\" class=\"img\" src=\"http:\/\/sciencewerke.local\/wp-content\/uploads\/2026\/04\/img-1-2.webp\"\/><br \/>\n<\/figure>\n<p><\/p>\n<p class=\"text-justify\" style=\"font-size: 20px;\"><strong><br \/>\n                            Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst<\/strong><\/p>\n<p class=\"text-justify\">Many researchers are unaware that photoconversion of DAPI and Hoechst can<br \/>\n                        cause nuclear stains to fluoresce in the FITC and Cy\u00ae3 channels.<\/p>\n<p class=\"text-justify\">Learn about the different ways to avoid DAPI photoconversion.<\/p>\n<p><a href=\"https:\/\/biotium.com\/tech-tips\/tech-tip-avoiding-artifacts-from-uv-photoconversion-of-dapi-and-hoechst\/\" onmouseout=\"this.style.textDecoration='none'\" onmouseover=\"this.style.textDecoration='underline'\" style=\"text-decoration: none; color: darkblue;\"><br \/>\n                        Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst<br \/>\n                    <\/a><\/p>\n<p>\n<img decoding=\"async\" alt=\"\" src=\"http:\/\/sciencewerke.local\/wp-content\/uploads\/2026\/04\/img-1-1.webp\"\/><\/p>\n<p class=\"text-justify\" style=\"font-style: italic;\">Photoconversion of DAPI to green and red fluorescence after exposure to<br \/>\n                            UV<br \/>\n                            light. Fixed<br \/>\n                            and permeabilized HeLa cells stained with DAPI were imaged by confocal microscopy<br \/>\n                            before or after exposure to mercury lamp excitation through the DAPI filter cube for 3<br \/>\n                            minutes.<\/p>\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst Many researchers are unaware that photoconversion of DAPI and Hoechst can cause nuclear stains to fluoresce in the FITC and Cy\u00ae3 channels. Learn about the different ways to avoid DAPI photoconversion. Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst Photoconversion of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1921,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[164],"tags":[],"class_list":["post-2686","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-164"],"_links":{"self":[{"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/posts\/2686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/comments?post=2686"}],"version-history":[{"count":1,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/posts\/2686\/revisions"}],"predecessor-version":[{"id":2751,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/posts\/2686\/revisions\/2751"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/media\/1921"}],"wp:attachment":[{"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/media?parent=2686"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/categories?post=2686"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pm11.corsivalab.xyz\/sciencewerke\/wp-json\/wp\/v2\/tags?post=2686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}