Introduction

The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a multi-day time-lapse, the LS850 offers a whole new world of walk-away automated microscopy!

Features and Benefits

 Images, tiled images, Z-stacks, time-lapse series, and videos recorded directly to your computer
 Bright field and phase contrast transmitted illumination
 LED excitation and high performance optics provide diffraction-limited (theoretical maximum) resolution
 Multi-OS software allows set-up and control across any locations, including microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers and custom arrays
 High speed stage with single digit micron precision and accuracy
 Long range motorized Z for autofocus and Z-stacks down to 100 nm
 Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and gloveboxes
 Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry GFP
 Open source Python control code available
 Objective compatibility with standard lenses and available with optional automated 4-objective turret

Like all of microscopes, the LS850 utilizes a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 590 nm LED light sources. The 5-filter set is comprised of 3 excitation filters, 1 triple-band dichroic, and 1 triple-band emission filter. The LS850 use a 12 bit monochrome CMOS sensor.

Click here for LS850 Microscope Product Brochure.

Excitation and Emission Profiles of LS850 filters

Description
Specifications
LED Excitation: 405 nm, 488 nm, and 589 nm
Fluorescence Filters: Blue: Excitation 370-410 nm, Emission 429-462 nm
Green: Excitation 473-491 nm, Emission 502-561 nm
Red: Excitation 580-598 nm, Emission 612-680 nm
Transmitted Modes: Bright Field, Phase Contrast
Objectives: 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil)
Camera: High Sensitivity Monochrome CMOS BSI Sensor; 5 MP 12-Bit
Image Formats JPG, BMP, TIF, or PNG
Image Size Adjustable up to 2100 x 2100 pixels
Video Rates: 25 FPS (exposure limited)
Automated XY Stage: 120 mm x 80mm travel, 1-3 micron positional repeatability
Throughput: 96-well, 3 channel fluorescence, w/o autofocus – 6 minutes 30 seconds
Motorized Z: 14mm travel, 100 nm step, image-based autofocus, Z-stacks
Control Software: LumaviewPro, multi operating system application
Computer Requirements: Windows 10, 11; Core i7, 512GB SSD, 8GB
RAM MacOS with M1; 512GB SSD, 8GB RAM
Linux Debian Distributions: 512GB SSD, 8GB RAM
Power Requirements: 80-264 V, 50-60 Hz 10W typical, 40W Max
Integration: Python source under the MIT Open Source License
Dimensions: 39.8 cm W x 29.7 cm D x 29.7 cm H
[15.6 in W x 11.6 in D x 11.6 in H]
Weight: 10 Kg/22lbs
Operating Conditions: 0°C – 42°C, 5% – 95% RH non-condensing