It leverages a novel PCR-free methodology for in situ break capture and sequencing by NGS, revealing the breaks induced by any nuclease-based genome editing system with high precision.

INDUCE-seq was built from the ground up to address a key unmet need in the accurate and rapid measurement of off-targets induced by gene editing. It is the first unbiased cell-based solution that is free from PCR induced biases that distort measurements, has broad compatibility with a wide range of therapeutically relevant cells, and applicable to any nuclease-based gene editing system. INDUCE-seq provides data-driven and actionable insights to accelerate research & development, pre-clinical and clinical stages gene editing programs.

Why INDUCE-seq?

• Whole genome, cell-based assay detecting on- and off-target double strand breaks induced by gene editing
• Unbiased (PCR-free) assay producing reproducible and accurate results
• Broad compatibility with any nuclease and a range of cell inputs, (adherent or suspension cells, iPSCs, primary cells, tissue)
• Scalable workflow built for automation

Gene Editing Applications

• Off-target assessment (specificity)
• On–target mechanism
• Kinetic analysis of nuclease/editing system
• Guide contamination screening
• Nuclease development
• Optimization of editing strategy (delivery, cell model, editing modality, modifying DNA repair, HDR)

INDUCE-seq is a cell agnostic platform to identify on- and off-target gene editing. Click here to find out more: https://www.brokenstringbio.com/cell-types-for-induce-seq/

How INDUCE-seq® works