Mito Flipper-TR® is a fluorescent probe that specifically targets the membrane of mitochondria of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains a mitochondria targeting motive as well as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore.

Mito Flipper-TR® spontaneously labels mitochondria of live cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm.

A detailed protocol for staining and FLIM imaging can be found in the datasheet. Probe quantity allows 35 – 150 staining experiments.*

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

Mito Flipper-TR® probe is distributed by Spirochrome under an exclusive license from the University of Geneva, Switzerland and was developed by the NCCR Chemical Biology.