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Genomic Solutions
Introduction Description
Introduction

Powered by helicase-driven processivity, uMRT brings greater coverage and sensitivity to direct RNA sequencing workflows, revealing longer transcripts, modification patterns, and transcript structures that other RTs simply miss.

Why Choose the uMRT Direct Kit?

  1. Higher CPMs & Longer Read Lengths
    This kit delivers >10% higher mapped reads from ≥ 2 kb RNAs. uMRT works at 30°C, which preserves the integrity of longer transcripts.
  2. Generate Higher cDNA Yields from your Sample
    uMRT efficiently converts even low-abundance, structured transcripts, increasing long-read cDNA yields > 25% and improving transcript-level quantification.
  3. Optimized for Native RNA
    uMRT’s high processivity and gentle 30 °C reaction temperature preserves full length, RNA integrity and base modifications—making it ideal for studying epitranscriptomic marks, RNA dynamics, and full-length isoforms.
  4. All-in-One Simplicity
    Eliminate the need to source multiple enzymes and reagents from different vendors. Our 6 & 48 reaction kits and standardized, validated workflow mean you no longer have to order multiple reagents from different vendors in quantities that may not match your experimental requirements.
Description

See the data: longer reads, more novel transcripts

Compared to the existing ONTR protocol, the uMRT Direct
RNA-Seq Kit shows:

  • 5–10% longer read lengths (with big gains >2 kb)
  • 25–50% higher cDNA yield
  • 1–2% more total genes and 15–20% more novel transcripts (NIC/NNIC)
  • 25–30% more intron retention events

 

Comparison: ONT Standard vs uMRT Direct

 

Kit Components:

  • UltraMarathonRT® (20 U/μL)
  • 2x RT Reaction Buffer
  • dNTP mix (10 mM each)
  • RNA Conditioner
  • 5x Ligation Buffer Q
  • DNA Ligase (2,000,000 U/mL)
  • RNase Inhibitor (40 U/uL)
  • Quenching Buffer
  • Nuclease-free Water

Catalog# R1008S (6 rxns), R1008M (48 rxns)


Click here for the product brochure.