The UltraMarathonRT® Two-Step RT-qPCR Kit ensures unmatched data reliability by providing consistent C t values regardless of primer type, RNA complexity, or GC content, eliminating biases and ensuring accurate quantification.
Unmatched Sensitivity: Enhancing and Streamlining Gene Expression Analysis
The UltraMarathonRT® Two-Step RT-qPCR Kit provides the most sensitive and reliable quantitation of gene expression on the market. Since the UltraMarathonRT enzyme is unphased by GC stretches and secondary structures of RNA templates, this RT-qPCR kit provides consistent C t values regardless of primer location ensuring accurate, reliable results each time.
Reliable, Precise, and Timely
With high inhibitor tolerance and a streamlined protocol that delivers results in under an hour, this versatile kit is ideal for RNA-seq validation, small RNA quantification, alternative splicing analysis, and CRISPR experiment evaluation.
Key Features
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- Data Reliability: Provides consistent C t value regardless of primer type (e.g. gene specific, oligo(dT) or randomer) or location. Other RT-qPCR kits will provide widely different C t values if primers are in high vs low GC content areas, whereas the UltraMarathonRT® Two-Step RT-qPCR Kit will provide consistent C t values regardless of location.
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- Robust Performance: Achieve exceptional sensitivity and efficiency, ideal for detecting low abundance RNA, viral RNA, non-coding RNAs, long RNAs, and even highly structured RNAs. This makes it a versatile tool for a wide range of molecular biology applications.
- High Inhibitor Tolerance: Consistent Ct values are maintained in the presence of inhibitory compounds potentially found in biological sample preparation such as ethanol (resistant to 10%), isopropanol (resistant to 5%), TRIzol (resistant to 0.7%), Formalin (resistant to 0.0025%), Ammonium Acetate (resistant to 50mM), Bile Salts (resistant to 0.05%), and Hematin (resistant to 10µM).
- Broad Dynamic Range: Capable of detecting RNA across a wide spectrum of input concentrations ranging from 0.1 pg to 2 µg, providing unparalleled flexibility in experimental design.
- Minimal Experimental Time: An optimized protocol allows researchers to generate complete RT-qPCR data in under 1 hour.
- Preserves RNA Template Integrity: UltraMarathonRT has intrinsic helicase activity for RNA structure unwinding, making the high temperature reaction conditions utilized by other RTs totally unnecessary.
- Ideal Companion for uMRT RNA-seq Validation: Only the UltraMarathonRT® Two-Step RT-qPCR Kit can reliably validate novel genes detected in uMRT RNA-seq data sets (genes that are undetected by other RTs).
- Dye-Based Detection: Incorporates reliable dye-based technology to facilitate accurate and reproducible real-time monitoring of RNA levels.