Pump System
A versatile Pump System for the
simulation of
physiological systems with defined shear stress:
mimicking blood vessels, endothelial cells under flow,
rolling and adhesion assays, perfusion of cells, spheroids,
and organoids for optimal nutrition.
Introduction
A Pump System for Long-Term Cell Culture Under Flow to Mimic Physiologic Conditions
- Establish in vivo-like conditions for the cultivation of cells under flow (e.g., endothelial cells for the simulation of blood vessels)
- Simulate defined laminar flow types (unidirectional, pulsatile, and oscillatory flow) occurring in different tissues and settings
- Compatible with µ-Slides with Luer adapters, to be used with all cell culture incubators, all inverted microscopes, and ibidi Stage Top Incubators
System Includes:
Applications
Many cell types experience moving fluids, such as vascular and lymphatic endothelial cells or kidney and lung epithelial cells. This liquid flow causes shear stress, a mechanical force that significantly impacts the cells’ physiological behavior, structural properties, and function. Cultivating cells under flow in vitro enables studying endothelial and epithelial cells in a more physiological, in vivo-like environment.
- Long-term cell culture under flow with defined shear stress values (e.g., endothelium, kidney, or biofilm).
- Simulation of defined continuous and pulsatile laminar flow, and oscillatory flow with defined shear stress to study cells in a more physiological environment.
- Rolling and adhesion assays using suspended cells (e.g., T cells) on substrates.
- Transmigration and invasion studies.
- Perfusion of single cells, spheroids, and organoids in 2D and 3D for optimal nutrition.
- Live cell imaging and immunofluorescence assays for analyzing shear stress response.
MV 20: The ibidi Pump System
Description
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