The PIF Supplement is used with buffered peptone water (BPW) in food microbiology for the enrichment of Enterobacteriaceae, particularly Salmonella spp. and Cronobacter spp.. It is adapted for matrices with a high level of background flora, such as lactic acid bacteria, bifidobacteria, and other Gram-positive bacteria. It is especially suitable for the detecton of Salmonella spp. and Cronobacter spp. in powdered infant formula with and without probiotics, ingredients for infant formula, and environmental samples of production areas.
Principle
The PIF Supplement is a special mix of components, which allows the inhibition of background flora
and, when associated with BPW, optimized recovery of Enterobacteriaceae, in particular
Salmonella
and Cronobacter.
Theoretical Composition
The PIF Supplement is supplied as a specific colored mix in 2 g amounts
Reconstitution Ratio
Dissolve 2 g of PIF Supplement in 40 ml of 1:1 ethanol/sterile distilled water solutiondirectly in
the plastic vial Mix, heating if necessary, until a homogeneous suspension is obtained
Enrichment Protocol – Preparation Table
Storage
Store dehydrated at 2-8 ⁰C in a carefully sealed package in a dry and dark place. The PIF Supplement
is stable for 6 weeks at 2-8 ⁰C in a dark place once reconstituted
References
– NF Validation BRD 07/06-07/04
– NF Validation BRD 07/11-12/05
– NF Validation BRD 07/23-01/13
– NF Validation BRD 07/22-05/12
– AOAC 082003