PCA (Plate Count Agar)
PCA (Plate Count Agar) Agar medium used to enumerate of the total aerobic microflora (total viable organisms) of production process and food products according to ISO Standard.
Description
Principle
PCA (Plate Count Agar) The nutrient substances provided by peptone, growth factors contained n yeast extract
and the use of glucose as an energy source promote the growth of most aerobic bacteria.
Theoretical Composition
| Enzymatic casein digest | 5 g |
| Yeast extract | 2.5 g |
| Glucose anhydrous | 1 g |
| Agar | 12 g |
| Distilled water | 1,000 ml |
| Final pH at 25 ⁰C = 7.0 ± 0.2 |
Reconstitution Ratio
20.5 g/L (500 g of powder makes 24.4 L of medium)
Storage
Store dehydrated medium at 15-25 ⁰C in carefully sealed bottles in a cool, dry place
References
– ISO 4833-1:2013
– ISO 4833-2:2013
– ISO 17410:2019