PCA (Plate Count Agar) without Dextrose
PCA (Plate Count Agar) without Dextrose Agar medium, also known as Yeast Extract Agar (YEA), for the enumeration of waterborne microorganisms using an aerobic colony count at 22 °C and 36 °C according to ISO Standard.
Description
Principle
The medium contains tryptone and yeast extract which promote the growth of most bacteria.
Theoretical Composition
| Tryptone | 6 g |
| Yeast extract | 3 g |
| Agar | 12 g |
| Destilled water | 1,000 ml |
| Final pH at 25 ⁰C = 7.2 ± 0.2 |
Reconstitution Ratio
21 g/L (500 g of powder makes 23.8 L of medium)
Storage
Store dehydrated medium at 15-25 ⁰C in carefully sealed bottles in a cool, dry place
References
– ISO 6222:1999
– ISO 11133:2014
– ISO 11133/A2:2020