The patented KASP™ PCR-based genotyping assay is a homogeneous, fluorescence (FRET) based assay that enables accurate bi-allelic discrimination of known SNPs and InDels. Unlike other PCR based genotyping assays, KASP requires no labelling of the target-specific primers / probes, giving it a clear cost advantage. It also allows greater flexibility for assay design, which translates into a higher overall success rate and the unique ability to genotype large / mega InDels.
KASP™ genotyping assays are based on competitive allele-specific PCR and enable bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci. KASP-TF Master Mixes are formulated without Triton™ X-100. The SNP-specific KASP Assay mix and the universal KASP-TF Master Mix are added to DNA samples, a thermal cycling reaction is then performed, followed by an end-point fluorescent read.