Dual-Range™ BCA Protein Assay Kit is the most used protein assay method. It based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein in the 5–2,000 μg/mL concentration range. Dual-Range™ BCA Protein Assay Kit is compatible with many detergents but not compatible with reducing agents such as DTT,DTE, and 2- Mercaptoethanol etc.
Application:
Blot, ELISA, Immunoassay,
Short Description :
Dual-Range™ BCA Protein Assay Kit is the most used protein assay method. It based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein in the 5–2,000 μg/mL concentration range. Dual-Range™ BCA Protein Assay Kit is compatible with many detergents but not compatible with reducing agents such as DTT,DTE, and 2- Mercaptoethanol etc.
Description
The BCA protein assay is based on a reduction of Cu2+ to Cu+ by proteins in alkaline solution with a sensitive and specific colorimetric detection of Cu+ by bicinchoninic acid (BCA). Two molecules of BCA chelate with each Cu+ and making the reduced copper from apple green to purple complex with strong absorbance at 562 nm.
Special Characteristic :
- Broad linear range: with standard protocol for 20-2000 μg/mL and enhanced protocol for 5-250 μg/mL
- Compatible: unaffected by typical concentrations of most ionic and nonionic detergents
Quick Instructions:
