Buffered Peptone Water (BPW) Standard is used as diluent and nutritive enrichment broth for the resuscitation and growth of a wide variety of microorganisms including Cronobacter, Enterobacteriaceae, Escherichia coli, and Salmonella in all food products, animal feed products, and water samples according to ISO Standard.
Principle
The special selection of peptones allows a very high level of nutritivity. In combination with the
phosphate buffer’s maintenance of a steady pH and osmotic balance, thanks to the presence of
chloride sodium, BPW Standard offers favorable conditions for the recovery and growth of a wide
range of microorganisms from a variety of matrices.
Theoretical Composition
| Enzymatic casein digest | 10 g |
| Sodium chloride | 5 g |
| Potassium dihydrogenphosphate | 1.5 g |
| Disodium hydrogen phosphate | 3.5 g |
| Distilled water | 1,000 ml |
| Final pH at 25 ⁰C = 7.0 ± 0.2 |
Reconstitution Ratio
20 g/L (500 g of powder makes 25 L of medium)
Storage
Store dehydrated medium at 15-25 ⁰C in carefully sealed bottles in a dry and dark place
References
– ISO 11290-2:2017
– ISO 19250:2013
– ISO 21528-1:2017
– ISO 22964:2017
– ISO 6579-1:2017
– ISO 6887-1:2017
– ISO 6887-5:2010