These kits include a BactoView™ Dead Stain and BactoView™ Viability Green Counterstain for two-color staining of live and dead bacteria. The kit is suitable for both gram-positive and gram-negative strains.
BactoView™ Viability Kits include Biotium’s novel BactoView™ Stains for two-color staining of live and dead bacteria. BactoView™ Dead Stains are novel membrane-impermeant DNA binding dyes that selectively stain dead bacteria with compromised cell membranes. BactoView™ Viability Green Counterstain labels both live and dead bacteria with green fluorescence and is formulated for two-color staining with BactoView™ Dead.
Features
Assess viability in both gram-positive or gram-negative bacteria strains
BactoView™ Dead is highly selective for dead cells (available in red or far-red)
BactoView™ Viability Green Counterstain labels both live and dead bacteria
Quick 30-minute staining, no wash required
Kit Components
BactoView™ Dead 570/585 (red) or BactoView™ Dead 655/670 (far-red)
BactoView™ Viability Green Counterstain
Robust Viability Assessment for Gram-Positive and Gram-Negative Strains
The kits are available with your choice of dye combination for green/visible red or green/far-red fluorescence detection. The stains are fluorogenic for no-wash staining and can be used to stain cells in culture medium or buffer. Traditional vital nucleic acid dyes like propidium iodide or ethidium homodimer are efficiently excluded from live gram-negative bacteria, which have an outer membrane protecting the cell wall. But these dyes often are taken up by live gram-positive bacteria that lack an outer membrane, resulting in high background in live cells and poor live/dead discrimination. BactoView™ Dead Stains have novel chemical structures that are efficiently excluded from both gram-positive and gram-negative strains, for highly selective live/dead discrimination.
BactoView™ Dead Stains & Viability Kits
| Product Name | Ex/Em (nm) | Detection Channel | Size (500X in DMSO) | Catalog No |
|---|---|---|---|---|
| BactoView™ Dead 500/515 | 497/515 | FITC | 20 uL 100 uL |
40107-T 40107 |
| BactoView™ Dead 560/570 | 559/570 | Rhodamine, PI, PE | 20 uL 100 uL |
40108-T 40108 |
| BactoView™ Dead 570/585 | 572/583 | Rhodamine, PI, PE | 20 uL 100 uL |
40109-T 40109 |
| BactoView™ Dead 600/615 | 603/613 | Texas Red® or PE-Texas Red® | 20 uL 100 uL |
40110-T 40110 |
| BactoView™ Dead 655/670 | 653/671 | Cy®5, APC | 20 uL 100 uL |
40111-T 40111 |
| BactoView™ Dead 690/710 | 683/707 | Cy®5.5 | 20 uL 100 uL |
40112-T 40112 |
| BactoView™ Dead 760/780 | 759/780 | Cy®7, APC-Cy®7 | 20 uL 100 uL |
40113-T 40113 |
| BactoView™ Viability Kit (Green/Red) | Green (498/522)/Red (572/583) | FITC (Green)/Rhodamine, PI, PE (Red) | 1 kit | 32019 |
| BactoView™ Viability Kit (Green/Far-Red) | Green (498/522)/Far-Red (653/671) | FITC (Green)/Cy®5, APC (Far-Red) | 1 kit | 32020 |
Texas Red is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.
We also offer BactoView™ Live stains with green or red fluorescence. Note that BactoView™ Stains cannot be used to distinguish bacteria from eukaryotic cells, because they will stain other cell types as well. For staining mammalian cells, see our NucSpot® Nuclear Stains for live/dead discrimination or nuclear counterstaining of fixed mammalian cells. For live nuclear staining of mammalian cells, see our NucSpot® Live Stains.
Product Attributes
| Apoptosis/viability marker | Dead cell stain, All cell stain |
| For live or fixed cells | For live/intact cells |
| Detection method/readout | Fluorescence microscopy, Flow cytometry |
| Assay type/options | Endpoint assay |
| Colours | Green, Red, Far-red |
| Storage Conditions | Store at -10 to -35 °C, Protect from light |
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