The new QuantSeq with UDI V2 kits are proposed as a series of 3’ mRNA-Seq library preparation kits including Lexogen’s patented 12 nucleotide-long Unique Dual Indices (UDI), in five different sets (A1, A2, A3, A4 and B1). These sets are identical to the ones of the original version 1.

 Keep working with the gold standard – same QuantSeq original technology
 Addition of Lexogen’s patented 12 nt UDI, for optimal demultiplexing
 Enhanced robustness for all your challenging samples (including FFPE)
 Free data analysis pipeline
 UDI Add-on modules available separately

Compatibility

The UDI Add-on V2 modules are available for purchase separately, for existing kits without UDI (QuantSeq Flex, Reverse or Forward). They are delivered together with an improved PCR system, which can be seamlessly adapted to previous QuantSeq kits. For QuantSeq-Pool kits, you only need the UDI Set (without the PCR enzyme and buffer).

QuantSeq with UDI V2 kits can still be used with the existing modules:

 PCR Add-on Kit for Illumina 020.96 – to determine the number of amplification cycles, by qPCR
 Reamplification Add-on Kit 080.96 – to reamplify undercycled libraries (on-demand kit)
 QuantSeq UMI module 081.96 – to add UMI to your libraries
 Blocking modules to prevent amplification of over-represented RNA sequences:
 Human globin: 070.96
 Pig globin: 071.96
 Mouse BC1: 167.96

They are also compatible with library preparation kits from other vendors.